Four members of the same family are missing in the Tsum Valley.
Francis and Jen Comber are travelling with their two young daughters Rani and Neve.
Mr Comber's mother Dianne Comber said they were waiting on any news of the family.
"They were trekking in the Tsum Valley, in the earthquake area," she said.
"No one's heard anything from them yet."
The family is on a long trip through Asia, leaving Australia in June last year.
Liam Oliver, 18, was travelling with his aunt Dianne Coburn further North in the Annapurna Valley when the quake hit.
Both are listed missing on the Red Cross's Family Links site, on which people can log in and let others know they are alive.
His brother Darcy confirmed to Fairfax Media he was missing but declined to comment.
He posted on Twitter on Sunday morning that Liam had been travelling the Annapurna Circuit, a popular trek.
The teen attended Daylesford Secondary College and lists cafe Moor Please as his employer on Facebook.
More than 1800 people are estimated dead and an increasing number of Australians are missing following the disaster.
Canberra mother Kiki Korpinen has not heard from her mother Riitta Korpinen or her 21-year-old son Hamish McKee since Wednesday, and alerted authorities on Saturday in the wake of earthquake which has claimed more than 1800 lives.
The Canberra grandmother and grandson have been in Nepal for three weeks. Riitta Korpinen, who lectures on early childhood around the world, helped established a school within an orphanage where she trains.
Her grandson accompanied her to work at the orphanage, at Biratnagar. They later went to Kathmandu for a couple of days, and then to Pokhara, where they were staying when the earthquake struck.
Victorian reserve grade football player Richard Jaroszczuk, 22, is also listed as missing from base camp at Mount Everest.
In Tasmania, a male and female from Launceston have been reported missing, along with a woman from Burnie and a woman whose hometown has not been confirmed.
A Kingston woman was previously listed missing, but has been found alive and safe.
Two Port Macquarie trekkers are safe, having been on the Peak Potential Adventures trek.
Sam Blundell was one of two Port Macquarie men on a trek in the Himalayas, led by his father Shane Popher from Peak Potential Adventures, when the earthquake hit.
Peak Potential Adventures joint owner Darren Wise confirmed on Sunday that the 13 people on the trek, including the expedition leader, were safe and well.
Others missing include Zachary Sheridan, the younger brother of Packed to the Rafters star Hugh Sheridan, who reached out on social media to people to pray for his sibling.
According to his listing on the Red Cross Family Links site, the 20-year-old from Adelaide last contacted his family on April 22 from Namche Bazar, described as the gateway to Everest.
Also listed as missing is Sydney-born film industry executive Zyra McAuliffe whose credits include Changi and Crocodile Dundee in Los Angeles.
Her husband, cinematographer Greg De Marigny, said the 41-year-old had planned to go trekking but believed she was well away from the areas worst affected by the earthquake.
Photo journalist Athena Zelandonii is also listed on the website, last contacting family on April 20 from Langtang Valley. According to Ms Zelandonii's Facebook page, the 25-year-old left Australia for an open-ended trip through South East Asia in mid-February and she arrived in Nepal at the beginning of April.
Sydney man Matthew Graves, 24, is also listed as missing. His family last heard from him on April 24 when he was in Pokhara, 200km from Kathmandu.
Perth woman Ballantyne Paige Forder, 20, is believed to have been working in an orphanage in Kathmandu at the time of the quake. Her sister Amanda-Sue Markham has taken to Twitter in a desperate bid to locate her younger sister.
Others listed as missing include 27-year-old Brisbane man Adam Jay Murphy; Dianne Joy Coburn, 59, of Melbourne; Eden Row, 20, of Melbourne; Sydney-born Brisbane woman Samantha McGree, 25; and Taisha-Dayne Reed, 23, of Melbourne.
An Australian born man, 43-year-old Jason Upton, who has been living in the UK is also listed among the missing.
Aid agencies including Oxfam are already sending teams to Nepal to help in the aftermath of the country's deadliest earthquake in more than 80 years.
Oxfam Australia chief executive Helen Szoke says a group of disaster specialists from the UK is flying in with supplies.
"People are gathered in their thousands in open spaces and are scared, as there were several aftershocks," Dr Szoke said in a statement.
"Oxfam staff in Nepal, along with thousands of others, are sleeping outside tonight in football fields and other open spaces because they are the safest place to be."
The Department of Foreign of Affairs has been contacted for comment.
with The Age
If you are concerned for a relative or friend in Nepal, call the Department of Foreign Affairs and Trade 24-hour consular emergency line on 1300 555 135 within Australia or +61 2 6261 3305 from outside.
